Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes.
Identifieur interne : 001540 ( Main/Exploration ); précédent : 001539; suivant : 001541Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes.
Auteurs : Vanessa A. Thomas [États-Unis] ; Bryon S. Donohoe [États-Unis] ; Mi Li [États-Unis] ; Yunqiao Pu [États-Unis] ; Arthur J. Ragauskas [États-Unis] ; Rajeev Kumar [États-Unis] ; Thanh Yen Nguyen [États-Unis] ; Charles M. Cai [États-Unis] ; Charles E. Wyman [États-Unis]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2017.
Abstract
Background
Consolidated bioprocessing (CBP) by anaerobes, such as
Results
Coupling CELF fractionation of corn stover and
Conclusions
Adding THF to DA pretreatment (CELF) greatly enhanced deconstruction of corn stover and
DOI: 10.1186/s13068-017-0937-3
PubMed: 29213312
PubMed Central: PMC5707920
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by <i>Clostridium thermocellum</i> and fungal enzymes.</title><author><name sortKey="Thomas, Vanessa A" sort="Thomas, Vanessa A" uniqKey="Thomas V" first="Vanessa A" last="Thomas">Vanessa A. Thomas</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Donohoe, Bryon S" sort="Donohoe, Bryon S" uniqKey="Donohoe B" first="Bryon S" last="Donohoe">Bryon S. Donohoe</name><affiliation wicri:level="2"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>National Renewable Energy Laboratory, Golden</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><affiliation wicri:level="2"><nlm:affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><affiliation wicri:level="2"><nlm:affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><affiliation wicri:level="2"><nlm:affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical & Bimolecular Engineering, Center for Renewable Carbon and Department of Forestry, Wildlife, and Fisheries, University of Tennessee Knoxville, Knoxville, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical & Bimolecular Engineering, Center for Renewable Carbon and Department of Forestry, Wildlife, and Fisheries, University of Tennessee Knoxville, Knoxville</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Nguyen, Thanh Yen" sort="Nguyen, Thanh Yen" uniqKey="Nguyen T" first="Thanh Yen" last="Nguyen">Thanh Yen Nguyen</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Cai, Charles M" sort="Cai, Charles M" uniqKey="Cai C" first="Charles M" last="Cai">Charles M. Cai</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:29213312</idno><idno type="pmid">29213312</idno><idno type="doi">10.1186/s13068-017-0937-3</idno><idno type="pmc">PMC5707920</idno><idno type="wicri:Area/Main/Corpus">001047</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001047</idno><idno type="wicri:Area/Main/Curation">001047</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001047</idno><idno type="wicri:Area/Main/Exploration">001047</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by <i>Clostridium thermocellum</i> and fungal enzymes.</title><author><name sortKey="Thomas, Vanessa A" sort="Thomas, Vanessa A" uniqKey="Thomas V" first="Vanessa A" last="Thomas">Vanessa A. Thomas</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Donohoe, Bryon S" sort="Donohoe, Bryon S" uniqKey="Donohoe B" first="Bryon S" last="Donohoe">Bryon S. Donohoe</name><affiliation wicri:level="2"><nlm:affiliation>National Renewable Energy Laboratory, Golden, CO USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Colorado</region></placeName><wicri:cityArea>National Renewable Energy Laboratory, Golden</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><affiliation wicri:level="2"><nlm:affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><affiliation wicri:level="2"><nlm:affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><affiliation wicri:level="2"><nlm:affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical & Bimolecular Engineering, Center for Renewable Carbon and Department of Forestry, Wildlife, and Fisheries, University of Tennessee Knoxville, Knoxville, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>Department of Chemical & Bimolecular Engineering, Center for Renewable Carbon and Department of Forestry, Wildlife, and Fisheries, University of Tennessee Knoxville, Knoxville</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Nguyen, Thanh Yen" sort="Nguyen, Thanh Yen" uniqKey="Nguyen T" first="Thanh Yen" last="Nguyen">Thanh Yen Nguyen</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Cai, Charles M" sort="Cai, Charles M" uniqKey="Cai C" first="Charles M" last="Cai">Charles M. Cai</name><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><affiliation wicri:level="2"><nlm:affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</nlm:affiliation><country>États-Unis</country><placeName><region type="state">Tennessee</region></placeName><wicri:cityArea>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge</wicri:cityArea></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Consolidated bioprocessing (CBP) by anaerobes, such as </p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>Coupling CELF fractionation of corn stover and </p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>Adding THF to DA pretreatment (CELF) greatly enhanced deconstruction of corn stover and </p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29213312</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by <i>Clostridium thermocellum</i> and fungal enzymes.</ArticleTitle><Pagination><MedlinePgn>252</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-017-0937-3</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Consolidated bioprocessing (CBP) by anaerobes, such as <i>Clostridium thermocellum,</i> which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using fungal enzymes for biological conversion of lignocellulosic biomass. However, limited research has integrated CBP with real pretreated biomass, and understanding how pretreatment impacts subsequent deconstruction by CBP vs. fungal enzymes can provide valuable insights into CBP and suggest other novel biomass deconstruction strategies. This study focused on determining the effect of pretreatment by dilute sulfuric acid alone (DA) and with tetrahydrofuran (THF) addition via co-solvent-enhanced lignocellulosic fractionation (CELF) on deconstruction of corn stover and <i>Populus</i> with much different recalcitrance by <i>C. thermocellum</i> vs. fungal enzymes and changes in pretreated biomass related to these differences.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">Coupling CELF fractionation of corn stover and <i>Populus</i> with subsequent CBP by the anaerobe <i>C. thermocellum</i> completely solubilized polysaccharides left in the pretreated solids within only 48 h without adding enzymes. These results were better than those from the conventional DA followed by either CBP or fungal enzymes or CELF followed by fungal enzyme hydrolysis, especially at viable enzyme loadings. Enzyme adsorption on CELF-pretreated corn stover and CELF-pretreated <i>Populus</i> solids were virtually equal, while DA improved the enzyme accessibility for corn stover more than <i>Populus</i>. Confocal scanning light microscopy (CSLM), transmission electron microscopy (TEM), and NMR characterization of solids from both pretreatments revealed differences in cell wall structure and lignin composition, location, coalescence, and migration-enhanced digestibility of CELF-pretreated solids.</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">Adding THF to DA pretreatment (CELF) greatly enhanced deconstruction of corn stover and <i>Populus</i> by fungal enzymes and <i>C. thermocellum</i> CBP, and the CELF-CBP tandem was agnostic to feedstock recalcitrance. Composition measurements, material balances, cellulase adsorption, and CSLM and TEM imaging revealed adding THF enhanced the enzyme accessibility, cell wall fractures, and cellular dislocation and cell wall delamination. Overall, enhanced deconstruction of CELF solids by enzymes and particularly by <i>C. thermocellum</i> could be related to lignin removal and alteration, thereby pointing to these factors being key contributors to biomass recalcitrance as a barrier to low-cost biological conversion to sustainable fuels.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Thomas</LastName><ForeName>Vanessa A</ForeName><Initials>VA</Initials><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Donohoe</LastName><ForeName>Bryon S</ForeName><Initials>BS</Initials><AffiliationInfo><Affiliation>National Renewable Energy Laboratory, Golden, CO USA.</Affiliation><Identifier Source="ISNI">0000 0001 2199 3636</Identifier><Identifier Source="GRID">grid.419357.d</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Mi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pu</LastName><ForeName>Yunqiao</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ragauskas</LastName><ForeName>Arthur J</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory (ORNL), Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemical & Bimolecular Engineering, Center for Renewable Carbon and Department of Forestry, Wildlife, and Fisheries, University of Tennessee Knoxville, Knoxville, TN USA.</Affiliation><Identifier Source="ISNI">0000 0001 2315 1184</Identifier><Identifier Source="GRID">grid.411461.7</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nguyen</LastName><ForeName>Thanh Yen</ForeName><Initials>TY</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Charles M</ForeName><Initials>CM</Initials><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials><Identifier Source="ORCID">0000-0002-7985-2841</Identifier><AffiliationInfo><Affiliation>Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Department of Bioengineering, Bourns College of Engineering, University of California Riverside, Riverside, CA USA.</Affiliation><Identifier Source="ISNI">0000 0001 2222 1582</Identifier><Identifier Source="GRID">grid.266097.c</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>BioEnergy Science Center (BESC), Oak Ridge National Laboratory, Oak Ridge, TN USA.</Affiliation><Identifier Source="ISNI">0000 0004 0446 2659</Identifier><Identifier Source="GRID">grid.135519.a</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>11</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Consolidated bioprocessing</Keyword><Keyword MajorTopicYN="N">Fractionation</Keyword><Keyword MajorTopicYN="N">Recalcitrance</Keyword><Keyword MajorTopicYN="N">Sugar</Keyword><Keyword MajorTopicYN="N">Tetrahydrofuran</Keyword><Keyword MajorTopicYN="N">Yield</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>07</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>10</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>12</Month><Day>8</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29213312</ArticleId><ArticleId IdType="doi">10.1186/s13068-017-0937-3</ArticleId><ArticleId IdType="pii">937</ArticleId><ArticleId IdType="pmc">PMC5707920</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Biotechnol Biofuels. 2016 Jun 02;9:116</Citation><ArticleIdList><ArticleId IdType="pubmed">27257435</ArticleId></ArticleIdList></Reference><Reference><Citation>ChemSusChem. 2016 May 23;9(10 ):1090-5</Citation><ArticleIdList><ArticleId IdType="pubmed">27116696</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2015 Dec 18;8:214</Citation><ArticleIdList><ArticleId IdType="pubmed">26691693</ArticleId></ArticleIdList></Reference><Reference><Citation>Environ Sci Technol. 2014 Jul 1;48(13):7200-3</Citation><ArticleIdList><ArticleId IdType="pubmed">24934084</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2016 Aug 31;138(34):10869-78</Citation><ArticleIdList><ArticleId IdType="pubmed">27482599</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2007 Feb 9;315(5813):804-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17289988</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Biol Macromol. 2016 Oct;91:60-7</Citation><ArticleIdList><ArticleId IdType="pubmed">27235731</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Biotechnol. 2007 Apr;25(4):153-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17320227</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):2077-82</Citation><ArticleIdList><ArticleId IdType="pubmed">19188587</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2011 Oct 7;10(10):4365-72</Citation><ArticleIdList><ArticleId IdType="pubmed">21678892</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6300-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21444820</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2010 Jul;101(13):4980-91</Citation><ArticleIdList><ArticleId IdType="pubmed">20206505</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2005 Dec;96(18):1967-77</Citation><ArticleIdList><ArticleId IdType="pubmed">16112484</ArticleId></ArticleIdList></Reference><Reference><Citation>Chem Rev. 2016 Aug 24;116(16):9305-74</Citation><ArticleIdList><ArticleId IdType="pubmed">27459699</ArticleId></ArticleIdList></Reference><Reference><Citation>MBio. 2012 Dec 11;3(6):null</Citation><ArticleIdList><ArticleId IdType="pubmed">23232718</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2008 Dec 1;101(5):913-25</Citation><ArticleIdList><ArticleId IdType="pubmed">18781690</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2011 Sep;102(17):8040-5</Citation><ArticleIdList><ArticleId IdType="pubmed">21683579</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2010 Dec;101(24):9624-30</Citation><ArticleIdList><ArticleId IdType="pubmed">20708404</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Microbiol. 1956 Jan;4(1):39-45</Citation><ArticleIdList><ArticleId IdType="pubmed">13283538</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Jan 27;311(5760):484-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16439654</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2014 Jul;111(7):1341-53</Citation><ArticleIdList><ArticleId IdType="pubmed">24522973</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2014 May 16;344(6185):1246843</Citation><ArticleIdList><ArticleId IdType="pubmed">24833396</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2014 May 13;7:72</Citation><ArticleIdList><ArticleId IdType="pubmed">24917886</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Biotechnol. 2012 Jun;23(3):396-405</Citation><ArticleIdList><ArticleId IdType="pubmed">22176748</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2011 Dec;102(24):11072-9</Citation><ArticleIdList><ArticleId IdType="pubmed">21478012</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2006 Jul 5;94(4):611-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16673419</ArticleId></ArticleIdList></Reference><Reference><Citation>Carbohydr Res. 2014 Feb 19;385:45-57</Citation><ArticleIdList><ArticleId IdType="pubmed">24412507</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2012 Apr;109(4):1083-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22095526</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2011 Dec;102(24):11052-62</Citation><ArticleIdList><ArticleId IdType="pubmed">21816612</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Prog. 1999 Oct 1;15(5):777-793</Citation><ArticleIdList><ArticleId IdType="pubmed">10514248</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Adv. 2016 Feb 05;2(2):e1501254</Citation><ArticleIdList><ArticleId IdType="pubmed">26989779</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Biochem Eng Biotechnol. 2007;108:95-120</Citation><ArticleIdList><ArticleId IdType="pubmed">17594064</ArticleId></ArticleIdList></Reference><Reference><Citation>ChemSusChem. 2015 May 22;8(10):1716-25</Citation><ArticleIdList><ArticleId IdType="pubmed">25677100</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 1976 May 7;72:248-54</Citation><ArticleIdList><ArticleId IdType="pubmed">942051</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2014 Apr 09;7:57</Citation><ArticleIdList><ArticleId IdType="pubmed">24713111</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Prog. 2009 Mar-Apr;25(2):333-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19294662</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2012;908:31-47</Citation><ArticleIdList><ArticleId IdType="pubmed">22843387</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2012 Nov 23;338(6110):1055-60</Citation><ArticleIdList><ArticleId IdType="pubmed">23180856</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Biofuels. 2012 Jul 02;5(1):45</Citation><ArticleIdList><ArticleId IdType="pubmed">22747961</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 1987 Jan;29(1):92-100</Citation><ArticleIdList><ArticleId IdType="pubmed">18561134</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioresour Technol. 2005 Apr;96(6):673-86</Citation><ArticleIdList><ArticleId IdType="pubmed">15588770</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiol Mol Biol Rev. 2002 Sep;66(3):506-77, table of contents</Citation><ArticleIdList><ArticleId IdType="pubmed">12209002</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2013 Jan 23;61(3):646-54</Citation><ArticleIdList><ArticleId IdType="pubmed">23270516</ArticleId></ArticleIdList></Reference><Reference><Citation>Biotechnol Bioeng. 2009 Feb 1;102(2):457-67</Citation><ArticleIdList><ArticleId IdType="pubmed">18781688</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Biotechnol. 2007 Jun;18(3):237-45</Citation><ArticleIdList><ArticleId IdType="pubmed">17462879</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1991 Mar 15;251(4999):1318-23</Citation><ArticleIdList><ArticleId IdType="pubmed">17816186</ArticleId></ArticleIdList></Reference><Reference><Citation>Philos Trans R Soc Lond B Biol Sci. 2008 Feb 27;363(1492):815-30</Citation><ArticleIdList><ArticleId IdType="pubmed">17761468</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li><li>Colorado</li><li>Tennessee</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Thomas, Vanessa A" sort="Thomas, Vanessa A" uniqKey="Thomas V" first="Vanessa A" last="Thomas">Vanessa A. Thomas</name></region><name sortKey="Cai, Charles M" sort="Cai, Charles M" uniqKey="Cai C" first="Charles M" last="Cai">Charles M. Cai</name><name sortKey="Cai, Charles M" sort="Cai, Charles M" uniqKey="Cai C" first="Charles M" last="Cai">Charles M. Cai</name><name sortKey="Donohoe, Bryon S" sort="Donohoe, Bryon S" uniqKey="Donohoe B" first="Bryon S" last="Donohoe">Bryon S. Donohoe</name><name sortKey="Donohoe, Bryon S" sort="Donohoe, Bryon S" uniqKey="Donohoe B" first="Bryon S" last="Donohoe">Bryon S. Donohoe</name><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><name sortKey="Li, Mi" sort="Li, Mi" uniqKey="Li M" first="Mi" last="Li">Mi Li</name><name sortKey="Nguyen, Thanh Yen" sort="Nguyen, Thanh Yen" uniqKey="Nguyen T" first="Thanh Yen" last="Nguyen">Thanh Yen Nguyen</name><name sortKey="Nguyen, Thanh Yen" sort="Nguyen, Thanh Yen" uniqKey="Nguyen T" first="Thanh Yen" last="Nguyen">Thanh Yen Nguyen</name><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><name sortKey="Pu, Yunqiao" sort="Pu, Yunqiao" uniqKey="Pu Y" first="Yunqiao" last="Pu">Yunqiao Pu</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Ragauskas, Arthur J" sort="Ragauskas, Arthur J" uniqKey="Ragauskas A" first="Arthur J" last="Ragauskas">Arthur J. Ragauskas</name><name sortKey="Thomas, Vanessa A" sort="Thomas, Vanessa A" uniqKey="Thomas V" first="Vanessa A" last="Thomas">Vanessa A. Thomas</name><name sortKey="Thomas, Vanessa A" sort="Thomas, Vanessa A" uniqKey="Thomas V" first="Vanessa A" last="Thomas">Vanessa A. Thomas</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001540 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001540 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:29213312
|texte= Adding tetrahydrofuran to dilute acid pretreatment provides new insights into substrate changes that greatly enhance biomass deconstruction by Clostridium thermocellum and fungal enzymes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29213312" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |